Merck, G6511-5MG, Glutathione S-Transferase from equine liver

Name: Glutathione S-Transferase from equine liver
Brand: Merck
SKU: G6511-5MG

Size:5 mg, GST, Glutathione R-transferase, Glutathione S-alkenetransferase, Glutathione S-alkyltransferase, Glutathione S-aralkyltransferase, Glutathione S-aryltransferase, Glutathione S-epoxidetransferase, lyophilized powder, ≥25 units/mg protein

Available Catalog Numbers

G6511-10MG G6511-1MG G6511-25MG G6511-5MG

Catalog Number: G6511-5MG

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Category

Recombinant Enzymes

Pack Size5 mg

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Glutathione S-transferase (GST) is a major detoxification enzyme, and exists as multiple cytoplasmic and membrane-bound isozymes. These isozymes differ in their catalytic activity, as well as in their non-catalytic binding properties. Cytoplasmic isoforms of GST are encoded by five genes, namely α, θ, μ, σ and π. α, μ and π are the most abundant forms in mammals. Membrane bound GST forms are encoded by a single gene.
Biochem/physiol Actions
Glutathione S-transferases are a family of proteins that catalyze the conjugation of reduced glutathione with a variety of hydrophobic chemicals containing electrophilic centers.
Protein family catalyzing conjugation of reduced glutathione with various hydrophobic chemicals.
Glutathione S-transferase (GST) from equine liver has been used-

as a constituent of Tris buffer for incubation of human umbilical vein endothelial cells (HUVEC) with atracurium to assess the proliferation of HUVEC in the presence of atracurium
as a component of GSB stock solution to determine GSB (glutathione S-bimane) conjugate fluorescence intensity in intact Arabidopsis cells
as an enzyme standard in spectrophotometric assay to determine the activity of GST

Physical form
Lyophilized powder containing Tris, reduced glutathione and EDTA.
Analysis Note
Protein determined by biuret.
Purified and assayed by a modification of the method of Simons and Vander Jagt.
Enzymatic activities are based on the conjugation of reduced glutathione with a second substrate. The individual proteins generally have activity with more than one class of substrate.
Other Notes
One unit will conjugate 1.0 μmole of 1-chloro-2,4-dinitrobenzene with reduced glutathione per min at pH 6.5 at 25°C.

Specifications

General

UNSPSC Code	12352204
NACRES	NA.47
Enzyme Commission (EC) Number	2.5.1.18 ( BRENDA \| IUBMB )
MDL number	MFCD00131198
biological source	equine liver
form	lyophilized powder
specific activity	≥25 units/mg protein
mol wt	45-50 kDa
composition	Protein, ≥60%
storage temp.	−20°C
Quality Level	200

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Merck, G6511-5MG, Glutathione S-Transferase from equine liver

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