Merck, G8255-2KU, Glutamic-Pyruvic Transaminase from porcine heart

General description
Glutamic-pyruvic transaminase (GPT) is localized majorly in the liver.

Research area: Cell Signaling
Application
Glutamic-Pyruvic Transaminase from porcine heart has been used:
in the transamination assay with a racemic mixture of synthetic 3-methyl-2-oxoglutarate
to remove glutamate from the cell culture medium used for culturing human embryonic kidney (HEK293) cells
in the lactate-dehydrogenase (LD)-coupled alanine transaminase (ALT) reaction kinetic studies

Biochem/physiol Actions
Glutamic-pyruvic transaminase (GPT) mediates the transfer of the amino group to α-ketoglutarate from l-alanine which results in pyruvate and glutamate.Thereby GPT plays a key role in gluconeogenesis and amino acid metabolism. The role of GPT in various kinds of tumors has been reported.
Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.
Physical form
Lyophilized powder containing citrate and acetate
Preparation Note
Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons
Analysis Note
Protein determined by biuret
Other Notes
One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.