Merck, G8529-10KU, Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

Application
Glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides has been used:

in the coupled spectrophotometric assay for PGI (F6P to G6P reaction)
to study the starch time course using Nicotiana tabacum leaves
to determine the glucose content in the root and stem samples of Quercus velutina Lam. saplings
to assay glucose using a starch sample of wild-type and transgenic Arabidopsis whole seedlings

Biochem/physiol Actions
Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Glucose-6-phosphate dehydrogenase (G6PD) is expressed in all tissues and produces the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH), which aids the cells to prevent oxidative stress. Insufficiency of G6PD is an X-linked, hereditary genetic defect. It is caused due to a common mutation in the G6PD gene.
Physical form
Lyophilized powder containing Ficoll and Tris buffer salts
Other Notes
One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.