Merck, G8880-50UN, Glutaminase from Escherichia coli

General description
Glutaminase is an amidohydrolase. It has the catalytic domain in the N-terminal and the C-terminal extension possesses STAS domain.
Application
Glutaminase from E.coli has been used:
for the determination of glutamine from the cerebral cortex tissue extract
for immobilization on aminopropylsilylated controlled pore glass (CPG) for determination of glutamine
in combination with acivicin in in vitro melanoma cells and MCF-7 and OAW-42 cells for cell proliferation and invasiveness assay.

Biochem/physiol Actions
Glutaminase from Escherichia coli along with acivicin, a glutamine analog regulates proliferation and invasiveness of cancer cells. Bacterial glutaminase is implicated in increasing the life span of cancer patients by lowering the tumor burden.
Glutaminase catalyzes the conversion of glutamine to glutamate.
Physical form
Lyophilized powder containing potassium succinate and EDTA
Other Notes
One unit will deaminate 1.0 μmole of L-glutamine per min at pH 4.9 at 37 °C.