Merck, GE11-0033-99, His GraviTrap™</SUP>

General description
His GraviTrap™ is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Column gives fast and simple gravity-flow purifications without any need for a purification.

The ready to use column is prepacked with Ni Sepharose™ 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives.

His GraviTrap™ columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10 mL.
Features and Benefits
For fast and simple purification of histidine-tagged* proteins.
Convenient, prepacked gravity-flow column.
Allows direct purification of both clarified and unclarified cell lysates.
High binding capacity; approximately 40 mg of pure protein per column.
Compatible with a wide range of reducing agents, detergents, denaturants and other additives.

Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva