Description
General description
HiTrap® Chelating HP is prepacked with Chelating Sepharose™ High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.
Application
HiTrap® Chelating HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC™ System
Features and Benefits
For optimizing purification of histidine-tagged Proteins using different kinds of metal ions.
Conveniently charge with your metal of choice.
Iminidoacetic acid coupled to Sepharose™ High Performance.
Purifying Proteins with exposed histidine groups via metal ion complex formation.
Simple operation with a syringe, pump or chromatographic system such as ÄKTA® design or FPLC™ System.
Packed with Chelating Sepharose™ High Performance
Simple operation with a syringe, pump or chromatographic system such as ÄKTATMdesign or FPLCTM System
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
