Description
General description
GSTrap™ HP columns are prepacked with Glutathione Sepharose™ High Performance medium and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.
GSTrap™ HP 1-ml and 5-ml columns are prepacked with Glutathione Sepharose™ High Performance medium, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose™ High Performance. GST-tagged proteins are bound to the affinity medium, and impurities are removed by washing with binding buffer.
GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Features and Benefits
High resolution purification of GST-tagged proteins.
Mild elution conditions preserving protein antigenicity and function.
Fast, one-step purification.
Easy to use with syringe, pump or a chromatography system.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
GSTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
