Merck, GE17-6002-33, HiTrap®IEX Selection Kit

General description
HiTrap® IEX Selection Kit consists of seven HiTrap® columns, prepacked with different Sepharose™ Fast Flow ion exchange chromatography media for screening of the most appropriate media and conditions to use for specific application and development work.

Q, SP, DEAE, and CM Sepharose™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.Q and SP Sepharose™ XL media have long chains of dextran coupled to a robust, 6% highly cross-linked agarose matrix. The dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities. The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose™ Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose™ 4 Fast Flow (high sub) has a diethylaminopropyl group attached. After choosing the optimal medium, prepacked columns and bulk media are available for larger scale preparative work.
Features and Benefits
Fast and easy selection with seven different ion-exchange ligands on Sepharose™ Fast Flow and Sepharose™ XL, prepacked in 1 mL HiTrap® columns.
Offers a fast, easy and convenient way screen for the optimal media for a given application .
Excellent for optimization studies; buffer composition, pH, flow rate, sample loading, and elution scheme can be optimized with small sample quantities prior to scale-up.
Designed for use with a syringe, peristaltic pump, or chromatography system such as AKTA design.

Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol (Q, DEAE, ANX,CM), 20% Ethanol, 0.2 M Sodium Acetate (SP))
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva