Description
General description
GenomiPhi HY Ready-To-Go DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 40 to 60 μg yield from nanogram amounts of DNA sample.GenomiPhi HY Ready-To-Go DNA Amplification Kit is part of the Phi29 DNA polymerase family of products from Cytiva. It contains all of the components necessary for whole genome amplification by isothermal strand displacement amplification. The reaction mixture is provided as predispensed, single-dose, lyophilized cakes in either strips of 8 tubes, or 96-well plates.
Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample. Furthermore, amplification is carried out with very high fidelity due to Phi29 DNA polymerase proofreading activity. The average product length is over 10 kb.
GenomiPhi HY Ready-To-Go DNA Amplification Kit procedure is quick, simple and does not require a thermocycler. A typical DNA yield of 40 to 60 µg DNA can be achieved in four hours from only 10 ng of genomic DNA input. The starting material for GenomiPhi reactions can be purified DNA from any commercial kit or homebrew method, or a nonpurified cell lysate may be used. Protocols for the amplification of DNA from blood lysates and dried blood spots are included in the manual.
GenomiPhi amplified DNA is suitable for various applications such as genotyping, cloning, sequencing, and DNA archival.
Features and Benefits
Representative amplification of the whole genome.
Preformulated, predispensed, single-dose, lyophilized cakes for rapid reaction set-up and greater reproducibility.
Quick and simple automation-friendly protocol; no thermal cycler required.
Shipping and storage at room temperature provides easy access and reduced carbon footprint.
Yields high-quality DNA (e.g. for high-throughput genotyping and DNA archival).
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
S-re the kit at ambient temperature. Once opened, completely reseal the pouch containing the Ready---Go GenomiPhi V3 DNA amplification kit cakes. - ensure maximum shelf life and optimal performance, s-rage in a desicca-r is recommended. S-re reconstituted Control DNA (Lambda) at -20°C. Once opened, s-re 2× denaturation buffer at 2–8°C.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
GenomiPhi
On packaging and insert:
For use only as licensed by Qiagen GmbH. and Cytiva. The Phi 29 DNA polymerase may not be re-sold or used except in conjunction with the other components of this kit. See US patent numbers 5,854,033, 6,124,120, 6,143,495, 6,323,009, 5,576,204, and equivalent patents and patent applications in other countries.
On container:
Subject to proprietary rights of Cytiva. May not be re-sold or used except with the other components of this kit. See US patent numbers 5,576,204, and equivalent patents and patent applications in other countries.
GenomiPhi is a trademark of Cytiva
Ready-To-Go is a trademark of Cytiva
