Merck, GE27-5340-02, AutoSeq™G-50

General description
AutoSeq G-50 columns are specifically designed to remove fluorescent dye-terminators from cycle sequencing reactions prior to analysis on automated sequencers. Effective DNA purification is essential for high-quality results on automated DNA sequencing since residual dye-terminators can obscure data. AutoSeq G-50 consists of MicroSpin columns containing Sephadex G-50 DNA Grade F, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artefacts on salt-sensitive automated DNA sequencers.
Features and Benefits

• For rapid removal of unincorporated fluorescent dye-terminators from cycle sequencing reactions prior to automated DNA sequencing
• Columns are prepacked with Sephadex G-50 DNA Grade F
• Columns are pre-equilibrated in double-distilled water with 0.05% Kathon CG/ICP Biocide added as a preservative
• Ready-to-use columns enable sample application through to collection of purified product in less than 4 min
• 4 minutes

Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
AutoSeq is a trademark of Cytiva