Description
General description
Balanced salt solution (BSS) plays an important role in cell culture as they provide a buffering system to maintain the medium within the optimum pH range (7.2-7.6). It also serves as an irrigating, transporting, and diluting agent to maintain the osmotic balance between intra- and extracellular spaces. BSS provides cells with water, inorganic ions, and energy for normal cell metabolism.
Application
Hanks′ Balanced Salt Solution 10x has been used:
to culture cerebella slices from rats
in Giardia duodenalis isolation
as a component of the dissection media for the storage of corpus callosum slices from mice
Preparation Note
Supplement with 0.35 g/L sodium bicarbonate at 1×.