ApplicationHyaluronidase from bovine testes has been used for the digestion of breast tissue, heart tissue and oocytes for isolation of cells.
Suitable for use in preparation of single cell suspension for sequencing.
Biochem/physiol ActionsThese enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.
Other NotesOne unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
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