Description
General description
Tissue Inhibitors of Metalloproteinases (TIMPs) comprise a family of four inhibitors known as TIMP-1, TIMP-2, TIMP-3, and TIMP-4. Each member of the TIMP family has its own distinct profile of metalloproteinase inhibition. The matrix metalloproteinases (MMPs) are a family of zinc proteases involved in the breakdown of extracellular matrix (ECM) in normal physiological processes, such as embryonic development, tissue and bone remodeling, wound healing, tissue morphogenesis, angiogenesis, and tumor metastasis. MMPs are also known to be involved in the cleavage of cell surface receptors, the release of apoptotic ligands (such as the FAS ligand), cell proliferation, differentiation, and chemokine in/activation. The normal function of MMPs must be precisely regulated by their endogenous protein inhibitors, TIMPs . TIMPs inhibit MMPs by forming 1:1, non-covalent complexes with MMPs, thereby blocking access of substrates to the MMP catalytic site. Except for inhibition of active MMPs, TIMPs also exhibit several other biochemical and physiological/biological functions such as proMMP activation, cell growth promotion, matrix binding, inhibition of angiogenesis and the induction of apoptosis. Disruption of the MMP/TIMP balance can result in serious diseases such as arthritis, cardiovascular disorders, tumor growth and metastasis.
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 4 analytes in human cell culture supernatants.
Analytes included: TIMP-1, TIMP-2, TIMP-3, TIMP-4
Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.
Features and Benefits
Configurable Panel Options: Researchers can select specific TIMPs (e.g., TIMP-1, -2, -3, -4) to tailor the panel to their study needs.
Integrated MMP/TIMP Pathway Analysis: Designed to work synergistically with MILLIPLEX® MMP Panels for comprehensive profiling of matrix remodeling.
Designed for Cell/Tissue Culture Supernatants: Optimized for non-serum matrices, making it ideal for mechanistic studies in vitro.
All-in-One Kit Format: Includes all necessary reagents, reducing setup time and ensuring consistency across runs.
Useful in Apoptosis and Angiogenesis Research: TIMPs are involved in cell growth, matrix binding, and inhibition of angiogenesis, making this panel valuable for diverse biological studies.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
For research use only. Not for use in diagnostic procedures.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
