Merck, I3021, IGEPAL®CA-630

General description
IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes. It is chemically indistinguishable from Nonidet P-40.
Application
IGEPAL® CA-630 has been used:
as a component of grinding buffer along with lithium dodecyl sulphate, tris-HCl, EDTA, NaCl, sodium deoxycholate, dithiothreitol and thiourea in RNA extraction process
along with Tris- HCl in incubation of Tachyzoites cultured in vero cells
as a component of nuclear extract buffer D+ containing HEPES, glycerol, KCl and EDTA for gel shift analysis
as a component of PI buffer along with NaH₂PO₄ to wash slides in FISH (fluorescence in situ hybridization) procedure
for solubilizing and isolation of membrane proteins

Analysis Note
Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.
Other Notes
Nonionic, non-denaturing detergent
Legal Information
IGEPAL is a registered trademark of Solvay
Disclaimer
May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.