Merck, I3911-1BO, Protease Inhibitor Cocktail, Animal Component Free

General description
Components of this cocktail are of non-animal origin and no animal products were used in the component production process. All vessels and instruments used for the cocktail production are dedicated for animal component free production and have never encountered an animal product. Aprotinin used in this cocktail is a recombinant bovine protein expressed in plants (Nicotiana).
This protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, and acid-proteases, and aminopeptidases.
Application
Animal component free (ACF). Optimized and tested for general use. Protease Inhibitor Cocktail, Animal Component Free has been used to prevent protein lysis in saliva samples, peritoneal residential macrophage cells and in embryo culture medium.
Biochem/physiol Actions
Inhibits serine, cysteine, aspartic, and metalloproteases.
Physical form
Lyophilized powder that is water soluble.
The components of this ACF cocktail do not originate from animal sources and can thus be used in the production of animal free proteins.
Preparation Note
One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.

Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.
Other Notes
AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin