Merck, I8904-50UG, Insulin-like Growth Factor-II from mouse

General description
Recombinant Mouse IGF-II is produced from a DNA sequence encoding the mature IGF-II protein. Mouse IGF-II, a 67 amino acid protein, has a predicted molecular matrix of ~7.4 kDa. Mouse and human IGF-II share 91% sequence homology. Insulin-like growth factor II (also known as multiplication stimulating activity or MSA) and insulin-like growth factor I (IGF-I) belong to the family of insulin-like growth factors, which are structurally homologous to proinsulin. Mature IGF-I and IGF-II are highly conserved and share ~70% amino acid sequence identity. They have autocrine, paracrine, and endocrine functions.
Biochem/physiol Actions
Insulin-like growth factor II (IGF-II) is a potent mitogenic growth factor that mediates growth-promoting activities in embryonic development. IGF-II binds the IGF-II receptor with high affinity. IGF-I and IGF II are expressed in many tissues and cell types. IGF-II is mitogenic for a variety of cultured cells including human or chicken fibroblasts, mouse 3T3 cells, normal rat kidney.
Physical form
Lyophilized from a 0.2 μm filtered solution of 30% acetonitrile and 0.1% of TFA.
Analysis Note
The biological activity is measured in a serum-free cell proliferation assay using the human breast carcinoma cell line MCF-7.