Description
General description
Jagged-1 is a Notch ligand. The Notch signaling system regulates proliferation, differentiation, and apoptosis in many tissues, including hematopoietic tissue. Notch is a family of transmembrane receptors activated by ligands (Jagged-1, Jagged-2, and Delta-1) that are expressed on adjacent cells. Hematopoietic stem cells and early progenitors express Notch-1 and Notch-2, whereas primary stromal cultures have been shown to express Jagged-1, indicating that Jagged-1 may play a role in nurturing progenitor cells within the hematopoietic microenvironment. Cells engineered to express high levels of Jagged-1 caused a four-fold increase in formation of hematopoietic progenitor cell colonies. However, without the presence of other cytokines that stimulate proliferation and maturation, such as IL-3, IL-6, SCF and G-CSF, Jagged-1 actually decreased myeloid colony formation of CD34⁺ cells, suggesting that Jagged-1 modulates cytokine signals and helps preserve CD34⁺ cells in an immature state.
Notch signaling is a well conserved among the metazoans and is a regulator of growth, differentiation, and patterning processes. One of the ligands of Notch family receptors is Jagged. Mammalian Jagged family comprises of Jagged-1 and Jagged-2. Jagged proteins are expressed during the critical stages of embryonic and postnatal development. The Notch-Jagged signaling regulates the proliferation of endothelial cells, apoptosis, differentiation of hematopoietic progenitors, skeletal development and formation and differentiation of blood vessels. Notch-jagged pathway components play important role in the pathology of diseases like Alagille syndrome, CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy) and osteosarcoma. Anti-Jagged 1 antibody specifically recognizes rat Jagged-1.
Immunogen
recombinant rat Jagged 1 extracellular domain, expressed in NSO cells.
Application
Anti-Jagged 1 antibody may be used for ELISA at a working antibody concentration of 0.5-1.0 μg/ml. For immunoblotting, antibody concentration of 0.1-0.2 μg/ml is recommended. For flow cytometry, a concentration of 20 μg/ml (10 μL/10⁵ cells to 10⁶ cells) may be used. The antibody is suitable for neutralization assays (ND50 is ~2-10 μg/ml).
Physical form
lyophilized from a 0.2 μm filtered solution of phosphate buffered saline (PBS), pH 7.4, and 5% trehalose
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
