Merck, K1757-50UG, Keratinocyte Growth Factor human

General description
Keratinocyte Growth Factor(KGF) is a 18.9 kDa growth factor, spanning 163 amino acids, belongs to the fibroblast growth factor family. It includes a secretory signal sequence and shares 39% identity with FGF-Basic. It is expressed in a number of stromal fibroblast cell lines, mesenchymal cells but absent from normal glial cells and epithelial cell lines.
Application
Keratinocyte Growth Factor human has been used:
as a component of epithelial growth medium
as a positive control for antigen to determine the activity of the antibody
to analyze the influence of KGF associated with thermo-sterilized carboxymethyl chitosan (NOCCts) in the reduction of pericardial adhesions

Biochem/physiol Actions
Keratinocyte Growth Factor(KGF) stimulates proliferation and differentiation of keratinocytes and other cells in the epithelium. It induces chemotaxis of microvascular endothelial cells and neovascularization of rat cornea. It stimulates the hyaluronic acid synthesis in proliferating and migrating basal cells. KGF contributes to the survival of cells under stress conditions. KGF is highly expressed in injured and inflamed epithelial cells, thereby, promotes wound healing.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.
Analysis Note
Determined by a cell proliferation assay using 4MBr-5 cells.