Description
Application
KAPA Taq PCR Kit has been used in:
High throughput PCR
Amplification of low copy DNA templates
Multiplex PCR
Specific amplification of complex templates
RT-PCR
random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)
Polymerase chain reaction (PCR)
Genotyping
Biochem/physiol Actions
KAPA Taq PCR Kit, which contains KAPA Taq DNA Polymerase, is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart® DNA Polymerase have 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 10⁵ nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.
Features and Benefits
High performance:
Improved sensitivity, specificity, and yields
Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product
Quick Notes:
KAPA Taq DNA Polymerase can replace any commercial Taq DNA polymerase in an existing protocol.
The final MgCl₂ concentration may need to be optimized to account for differences in buffer formulation.
KAPA Taq Buffers contain MgCl₂at a final concentration of 1.5 mM. Buffer A is recommended as first approach and for applications requiring high yields. Buffer B is recommended for applications where high sensitivity is required (e.g. when the template is limiting). Both buffers may be evaluated to determine the buffer most suitable for a specific application.
The KAPA Taq PCR system is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C forshort-term use (up to 1 month). Return to -20°C for long term storage.
Analysis Note
Each batch of KAPA Taq DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230Assay). KAPA Taq Ready Mixes are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.
Other Notes
For Research Use Only. Not for use in diagnostic procedures.
Legal Information
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
