| NACRES | NA.85 |
|---|---|
| UNSPSC Code | 41106200 |
| grade | Molecular Biology |
| sterility | non-sterile |
| form | powder |
| composition | NaCl, 5 g/L |
| technique(s) | microbiological culture: suitable |
| pH | 6.8-7.2(2% solution) |
| application(s) | food and beveragesmicrobiology |
| storage temp. | room temp |
| suitability | nonselective for Escherichia coli |
| Quality Level | 200 |
Answer:Dissolve 20 g powder in one liter of distilled water and autoclave for 15 minutes at 121°C.
Answer:The Lennox formulation contains 5 g/L NaCl, lower than the commonly used Miller LB formulation.
Answer:No. The dehydrated powder is nonsterile and contains no specified antibiotic; sterilize the prepared broth and add selective agents separately.
Sigma LB Broth (Lennox) is a powdered microbiological culture medium for propagating suitable Escherichia coli strains in routine molecular biology workflows. The Lennox low-salt formulation yields approximately 10 g/L tryptone, 5 g/L yeast extract and 5 g/L sodium chloride when prepared at 20 g powder per liter. These nutrient sources support growth for bacterial cloning, plasmid production and recombinant expression workflows. The 250 g package is supplied as nonsterile dehydrated powder; it is not a ready-to-use liquid broth. Dissolve the appropriate amount in purified water and sterilize by autoclaving at 121°C for 15 minutes under the validated laboratory procedure. Antibiotics or other selective agents must be added separately when required, normally after the sterilized medium has cooled to an appropriate temperature. Lennox broth has a lower sodium chloride content than Miller LB, so selection can influence growth and antibiotic selection performance. Check pH, sterility and growth suitability before use, particularly when comparing with other LB formulations.
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