Merck, L3397-1KG, LB Broth (Luria low salt)

General description
Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation.
Application
LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique.
Features and Benefits
Luria′s low salt LB powder provides:
Standard formulation
A budget-friendly alternative to liquid
Easy scale-up using larger package sizes

Preparation Note
1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.
Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).