Merck, L4277-250ML, Lipase from Aspergillus oryzae

General description
Lipase from Aspergillus Oryzae is a carboxylesterase and belongs to the α/β-hydrolase family. It comprises a lid domain, hinge domain and a catalytic triad Ser?His?Asp/Glu. The three-dimensional structure is a α/β hydrolase fold which is very similar as that of esterase enzyme.
Application
Lipase from Aspergillus Oryzae has been used:

in the partial digestion of triacylglycerols (TAG)
in the digestion of (12-ricinoleoylricinoleoyl)diricinoleoylglycerol (RRRR) present in castor oil
in the hydrolysis of linseed oil

Biochem/physiol Actions
Lipase catalyzes the hydrolysis of monoacylglycerols and diacylglycerols and its activity is inhibited by divalent actions. Aspergillus Oryzae lipase is an industrial enzyme with application in food, detergent and pharmaceutical industries. It is also used in immobilization studies for 1,3-dioleoyl-2-palmitoylglycerol (OPO) synthesis. Aspergillus Oryzae lipase may have potential in producing biodiesel from waste cooking oil.
Preparation Note
purified 1,3-specific lipase from Aspergillus Oryzae produced by submerged fermentation of a genetically modified Aspergillus Oryzae microorganism
Legal Information
A product of Novozyme corp.
Palatase is a registered trademark of Novozymes Corp.