Description
General description
LuminoCt® SYBR® Green qPCR ReadyMix is ready to use master mix that combines the performance enhancements of our JumpStart™ Taq antibody for hot start. Extensive design and validation of the ReadyMix chemistry has virtually eliminated the need for optimization when LuminoCt is used in conjunction with properly designed primers. Inclusion of JumpStart™ Taq antibody in the ReadyMix eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup.
Kit is designed to perform SYBR Green-based qPCR assays on commonly available real-time instrument platforms
ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays
Kit is not compatible with qPCR instruments that utilize glass capillary tubes
Application
LuminoCt® SYBR® Green qPCR ReadyMix™ has been used for fast quantitative polymerase chain reaction (qPCR) and quantitative RT-PCR amplifications. It has also been used in qPCR for measuring gene expression.
Features and Benefits
Assay results in as little as 25 minutes
Unsurpassed accuracy, precision, and sensitivity
Virtually eliminates the need to optimize assay parameters
Ideal for performing high-throughput, quantitative PCR applications
The master mix allows consistency from one reaction to the next
Reduced preparation time and reduced risk of contamination from multiple pipetting steps
The hot start mechanism using the JumpStart Taq antibody prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
LuminoCt® ReadyMixes require the use of small amplicons (This master mix is compatible with commercial primer sets, including TaqMan® assays
Packaging
Default reaction volume is 50 μL
100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL
Other Notes
At a minimum, use software to design primers for assays and ensure that qPCR amplicons are Sigma recommends designing primers and probes with Beacon Designer software
To order primers and probes from Sigma, visit: www.sigma.com/oligos
Legal Information
Eppendorf is a registered trademark of Eppendorf SE
JumpStart is a trademark of Sigma-Aldrich Co. LLC
LuminoCt is a registered trademark of Merck KGaA, Darmstadt, Germany
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
