Merck, L6632-5MU, Lipoxidase from Glycine max (soybean)

Application
Lipoxidase, or lipoxygenase, from Glycine max (soybean) has been used for the modification of low density lipoprotein, isolated from human plasma.
The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).
Biochem/physiol Actions
Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.
Physical form
Suspension in 2.3 M (NH₄)₂SO₄ solution, pH approx. 6.0
Preparation Note
Prepared by ion exchange chromatography and hydrophobic interaction chromatography.
Analysis Note
Protein determined by biuret.
Other Notes
One unit will cause an increase in A₂₃₄ of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A₂₃₄ unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.