Merck, L9895-2MG, Lectin from Pseudomonas aeruginosa

Biochem/physiol Actions
PA-I has a major affinity for D-galactose and its derivatives. PA-I agglutinates all types of human and animal erythrocytes and interacts also with unicellular protozoa, algae and bacteria. PA-I induces mitogenic stimulation in cultured human lymphocytes pretreated with neuraminidase.
Packaging
Package size based on protein content. Vial content based on protein by biuret.
Physical form
Lyophilized powder containing approx. 80% protein (biuret), balance primarily sodium citrate, plus traces of calcium chloride, magnesium chloride and manganese chloride
Preparation Note
Purified by affinity chromatography using a modification of Gilboa-Garber.¹
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution using phosphate buffered saline, pH 7.0. This activity is the lowest concentration to agglutinate a 2% suspension of neuraminidase treated human blood group A erythrocytes after one hr incubation at 25 °C.
Highly purified.