Description
General description
Lyophilized liposomes product series are freeze-dried liposomes with various types of lipids and wide range of zeta potentials and different properties. These products should be used by scientists who understand liposome formulation and have the proper equipment to check the size, separate non-encapsulated drugs and do the proper assays. Liposomes are extensively used to study the interaction of proteins, peptides and other molecules with the surface of a lipid membrane. One of the parameters that affects this interaction is the charge of the liposomal membrane. Liposomes are always made in aqueous environment and they are sized to the desired size in liquid state using various methods such as high-pressure extrusion through nano sized pore track etch membranes. In rare occasions, liposomes are freeze dried and proliposomes are formed in the presence of a lyoprotectant such as trehalose. Using a lyoprotectant is necessary in order to maintain the size of the liposomes after rehydration.
Natural Phosphatidylcholine (PC) liposomes are made from natural lipids extracted from egg yolk, soybean, or tissues such as brain, heart, and liver. The Natural PC liposomes are available with and without cholesterol. Adding 30% cholesterol makes the liposomes serum stable and is necessary when the liposomes are used in the presence of serum.
Heart phosphatidylcholine (PC) contains 40% plasmalogen ether lipids. Ether lipids are more stable and do not get hydrolyzed. Heart PC is also composed of both saturated and unsaturated lipids including: 23% 16:0 lipid, 7% 18:0 lipid, 11% 18:1 lipid, 43% 18:2 lipid and 6% 20:4 lipid.
Application
Liposomes are formed upon hydration of the lyophilized formulation. If the lyophilized liposomes are hydrated with solution containing a water-soluble drug, then a large percentage of the drug will stay outside of the liposomes and in non-encapsulated form. It is advised to use a micro dialysis cassette or a spin column using the right-side beads (depending on the size of your drug) and separate the drug encapsulated liposomes from free drug and perform the drug assay in order to calculate the encapsulation efficiency.
Lyophilized liposomes are mainly recommended to be used with drugs that have a short life in aqueous solution mainly due to hydrolysis. After adding the solution of the drug to lyophilized liposomes, the liposomes should be used immediately.
Lyophilized liposomes products should be used by scientists who understand liposome formulation and have the proper equipment in order to check the size, separate the non-encapsulated drug and do the proper assays.
Trehalose is used as a lyoprotectant in all freeze-dried liposome formulation. The size distribution after hydration of the freeze-dried formulation will be around 100 nm.
Freeze-dried liposomes should be kept at -20°C.
