Merck, M2786-25MG, 3-Maleimidobenzoic acid N-hydroxysuccinimide ester

General description
may require addition of solvent to coupling buffer to at least 5% to maintain solubility
Application
3-Maleimidobenzoic acid N-hydroxysuccinimide ester (MBS) has been used as a protein cross-linker. It has been used for the stabilization of actin filaments in plant tissue samples.
3-Maleimidobenzoic acid N-hydroxysuccinimide ester has been used:
in the fixation of pollen in Arabidopis
as a component of Murashige and Skoog (MS) medium to stabilize F-actin in Eucalyptus root hairs
in the fixation of F-actin labelled pollen tubes of Lilium davidii

Heterobifunctional crosslinking reagent reactive toward primary amine and sulfhydryl. Typically, coupled initially to molecules containing primary amines by amide bonds buffered at pH 7.0, followed by coupling to compounds containing sulfhydryl by thioether at same pH. Useful for preparation of enzyme immunoconjugates and hapten carrier molecule conjugates.
Biochem/physiol Actions
3-Maleimidobenzoic acid N-hydroxysuccinimide is used as a fixative for the preservation of pollen tube F-actin distribution. It imposes less damage when used on pollen tubes.
Other Notes
The aryl maleimide is less stable than alkyl maleimide linkers.