Description
General description
Fibroblast growth factor receptor 4 (UniProt: P22455; also known as EC: 2.7.10.1, FGFR-4, CD334) is encoded by the FGRF4 (also known as JTK2, TKF) gene (Gene ID: 2264) in human. FGFR-4 is a highly conserved, single-pass type I membrane protein with tyrosine kinase activity that serves as a cell surface receptor for fibroblast growth factors and regulates cell proliferation differentiation, and migration. FGFR-4 is expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines. In the absence of a ligand, it is present as a monomer in an inactive conformation. Upon ligand binding it homodimerizes into an active conformation with autophosphorylation of tyrosine residues. FGFR-4 is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off in the mature form. It contains an extracellular domain (aa 22-369), a transmembrane domain (aa 370-390), and a cytoplasmic domain (aa 391-802). FGFR-4 undergoes N-glycosylation, and when not fully glycosylated, it can undergo proteasomal degradation. FGFR-4 signaling is down-regulated by receptor internalization and degradation. MMP14 is reported to promote internalization and degradation of FGFR-4. Mutations that lead to constitutive kinase activation or impair normal FGFR-4 inactivation lead to aberrant signaling leading to tumor growth. Over-expression of FGFR4 is reported in almost all human prostate cancers and its R388G mutation is associated with tumor progression. It leads to increased receptor stability and sustained receptor activation. (Ref.: Turkington, RC et al. (2014). Cell Death Dis. 5; e1046; Wang, J., et al. (2008). Neoplasia. 10(8); 847-856).
Immunogen
Cells transfected with human FGFR4.
Application
Quality Control Testing
Evaluated by Immunocytochemistry in HepG2 cells.
Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Fibroblast growth factor receptor 4 (FGFR4) in HepG2 cells.
Tested Applications
ELISA Analysis: A representative lot detected Fibroblast growth factor receptor 4 (FGFR4) in ELISA applications.
Inhibition Assay:: A representative lot inhibited growth, replication, and metastasis in RH30 cells.
Immunotoxin: A representative lot of this antibody displayed immunotoxin activity as antibody-drug conjugate with Monomethyl auristatin F or Duocamycin DM.
Flow Cytometry Analysis: A representative lot detected Fibroblast growth factor receptor 4 (FGFR4) in Flow Cytometry applications.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone BT53 is a mouse monoclonal antibody that detects FGFR4. It targets an epitope within the extracellular domain.
Physical form
Purified mouse monoclonal antibody IgG2b in PBS without preservatives.
Preparation Note
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
