Description
General description
CD44 antigen (UniProt: P16070; also known as CDw44, Epican, Extracellular matrix receptor III (ECMR-III), GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1 (PGP-1), Phagocytic glycoprotein I (PGP-I)) is encoded by the CD44 (also known as LHR, MDU2, MDU3, MIC4) gene (Gene ID: 960) in human. CD44 is a Type 1 transmembrane glycoprotein involved in cell adhesion, migration, and proliferation. It also plays a role in lymphocyte activation, tumor metastasis, and wound healing. It is encoded by the CD44 gene, and it is expressed in a wide range of cell types. The protein′s structure includes a hyaluronic acid (HA) binding domain, a stem region, and a variable extracellular domain. Alternative splicing may produce 19 isoforms. CD44 splice variants (CD44v) play a role in tumorigenesis and malignancy. It is also implicated in various other diseases, including autoimmune disorders, cardiovascular diseases and fibrosis.
Immunogen
CHO cells transfected with PA16-tagged CD44v3 10.
Application
Quality Control Testing
Evaluated by Flow Cytometry in a CHO cells overexpressing CD44v3-10.
Flow Cytometry Analysis: 1.0 µg of this antibody detected CD44v6 in a one million CHO cells overexpressing CD44v3-10.
Tested Applications
Western Blotting Analysis: A representative lot detected CD44v5 in Western Blotting applications (Kudo, Y., et al. (2023). Antibodies (Basel). 12(2):31).
Immunohistochemistry Applications: A representative lot detected CD44v5 in Immunohistochemistry applications (Kudo, Y., et al. (2023). Antibodies (Basel). 12(2):31).
Flow Cytometry Analysis: A representative lot detected CD44v5 in Flow Cytometry applications (Kudo, Y., et al. (2023). Antibodies (Basel). 12(2):31).
ELISA Analysis: A representative lot detected CD44v5 in ELISA applications (Kudo, Y., et al. (2023). Antibodies (Basel). 12(2):31).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone C44Mab-3 is a mouse monoclonal antibody that detects CD44v5. It targets an epitope within 30 amino acids from the N-terminal half of the protein.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
