Description
General description
RE1-silencing transcription factor (also known as REST, Neural-restrictive silencer factor, X2 box repressor) is encoded by the REST (also known as NRSF, XBR) gene (Gene ID: 422623) in chicken. REST is a transcriptional repressor that acts by binding a DNA sequence element called the neuron-restrictive silencer element (NRSE). It is ubiquitously expressed, and higher expression is found in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes, and ovary. It is also found in undifferentiated neuronal progenitor cells and it is believed that it acts as a master negative regular of neurogenesis. REST is also required to repress endogenous neuronal target genes in non-neuronal tissues, and also to repress these genes in neural precursors. Expression of REST is reported to be cell cycle-dependent with reduced levels in the G2 phase. REST restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. REST also mediates repression by recruiting the BHC complex at RE1/NRSE sites, which acts by deacetylating and demethylating specific sites on histones and acting as a chromatin modifier. REST is shown to contain nine zinc finger domains, but a C-terminally truncated form is also produced by alternative splicing. Mutations in human REST gene can lead to Wilms tumors, a pediatric malignancy of the kidney that occurs due to uncontrolled multiplication of renal stem, stromal, and epithelial cells. ((Ref.: Chen, ZF., et al. (1998). Nat. Genet. 20(2); 136-142).
Immunogen
GST-tagged recombinant fragment corresponding to the first 181 amino acids (without the initiator methionine) from Chicken RE1 silencing transcription factor (REST).
Application
Quality Control Testing
Evaluated by Western Blotting in lysate from E14-Tg2a mouse embryonic stem cells.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected REST in lysate from E14-Tg2a mouse embryonic stem cells.
Tested Applications
Electrophoretic Mobility Shift Assay: A representative lot detected REST in Electrophoretic Mobility Shift Assay. (Kim, S.M., et al. (2006). Biochem Biophys Res Commun. 346(2):426-35).
Immunocytochemistry Analysis: A representative lot detected REST in Immunocytochemistry applications (Shimojo, M. et al. (2008). J Biol Chem. 283(50):34880-6).
Western Blotting Analysis: A representative lot detected REST in Western Blotting applications (Chen, Z.F., et al. (1998). Nat Genet. 20(2):136-42).
Immunoprecipitation Analysis: A representative lot immunoprecipitated REST in Immunoprecipitation applications (Shimojo, M. et al. (2008). J Biol Chem. 283(50):34880-6).
Immunohistochemistry Applications: A representative lot detected REST in Immunohistochemistry applications (Paquette, A.J., et al. (2000). Proc. Natl. Acad. Sci. USA. 97(22):12318-23).
Immunocytochemistry Analysis: A 1:25 dilution from a representative lot detected REST in NTERA-2 cell line.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-REST, clone 12C11, Cat. No. MABE1982, is a mouse monoclonal antibody that detects REST and is tested for use in Electrophoretic Mobility Shift Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Biochem/physiol Actions
Clone 12C11 is a mouse monoclonal antibody that detects RE1-silencing transcription factor (REST). It targets an epitope within the N-terminal region.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
