Merck, MABF2006-25UG, Anti-CHIKV CP Antibody, clone 5.5G9

General description
Purified inactivated capsid protein from CHIKV Mauritius strain (CHIKVMAU), challenged with live virus (CHIKVTHAI), followed by a final boost with inactivated antigen (CHIKVTHAI).
~30 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Application
Immunohistochemistry Analysis: A representative lot detected CHIKV CP in Immunohistochemistry applications (Goh, L.Y.H., et. al. (2015). J Gen Virol. 96(Pt 3):507-12).

Western Blotting Analysis: A representative lot detected CHIKV CP in Western Blotting applications (Goh, L.Y.H., et. al. (2015). Viruses. 7(6):2943-64).

ELISA Analysis: A representative lot detected CHIKV CP in ELISA applications (Goh, L.Y.H., et. al. (2015). J. Gen. Virol. 96(Pt 3):507-12).

Western Blotting Analysis: A 1:500 dilution from a representative lot detected CHIKV CP in C6/36 mosquito cell lysates either mock-infected (negative) or CHIKV-infected (positive). (Courtsey of Jody Peters, Ph.D.., University of Queensland, Australia).



Biochem/physiol Actions
Clone 5.5G9 specifically detects capsid protein in Chikungunya virus. It detects N-truncations (N1-4) and the full-length capsid protein.

Physical form
Purified mouse monoclonal antibody IgG2a in PBS without azide.

Preparation Note
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed to be mouse IgG2a by isotype testing.

Other Notes
Chikungunya virus.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.