Description
General description
Macrophage colony-stimulating factor 1 (UniProt: P07141; also known as CSF-1, MCSF) is encoded by the Csf1 (also known as Csfm) gene (Gene ID: 12977) in murine species. CSF-1 is a widely expressed, single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-32), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 33-492), a transmembrane domain (aa 493-515), and a cytoplasmic domain (aa 516-552). Following its synthesis, it can be cleaved into a 43 kDa subunit CSF1 and a processed secreted form. CSF-1 is a cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells and promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Its actions are mediated by a high affinity CSF1-receptor. Clone 5A1 is shown to bind and inhibit the biological functions of CSF-1. It is reported to inhibit colony formation of tissue mononuclear phagocyte colony-forming cells as well as the committed bone marrow stem cells for both granulocytes and monocytes. The extent of colony inhibition by this clone is dependent on the tissue origin of colony-forming cells. Two isoforms of CSF-1 have been described that are produced by alternative splicing. (Ref.: Wei, S., et al. (2006). J. Leukoc. Biol. 80(6); 1445-1453; Lokeshwar, BL., and Lin, H-S. (1988). J. Immunol. 41(2); 483-488).
Immunogen
Purified, serum-free, stage 4 CSF-1 from murine L cell-conditioned medium.
Application
Quality Control Testing
Evaluated by ELISA with recombinant mouse CSF-1.
ELISA Analysis: Serial dilutions of this antibody detected recombinant mouse CSF-1.
Tested Applications
Neutralizing: A representative lot neutralized the colony-stimulating activity of CSF-1.(Lokeshwar, B.L., et al. (1989). J Immunol Methods. 123(1):123-9).
ELISA Analysis: A representative lot detected CSF-1 in ELISA applications (Lokeshwar, B.L., et al. (1989). J Immunol Methods. 123(1):123-9).
Radioimmunoassay: A representative lot detected CSF-1 in Radioimmunoassay applications (Lokeshwar, B.L., et al. (1989). J Immunol Methods. 123(1):123-9).
Inhibition: A representative lot bound and inhibited the biologic function of CSF-1.(Lokeshwar, B.L., et al. (1989). J Immunol Methods. 123(1):123-9).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 5A1 is a rat monoclonal antibody that detects Macrophage colony-stimulating factor 1 (CSF-1).
Physical form
Purified rat monoclonal antibody IgG1 in PBS without azide.
Preparation Note
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
