Description
General description
Anthrax toxin receptor 2 (UniProt: P58335; also known as Capillary morphogenesis gene 2 protein, CMG-2) is encoded by the ANTRX2 (also known as CMG2) gene (Gene ID: 118429) in human. ANTRX2/CMG-2 is widely expressed, single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 34-318), a transmembrane domain (aa 319-341), and a cytoplasmic domain (aa 342-489). It shares about 58% amino acid identity with ANTXR1. Four isoforms of ANTRX2/CMG-2 have been described that are produced by alternative splicing. It is reported to be essential for cellular interactions with laminin and the extracellular matrix. It is an important regulator of collagen VI homeostasis. It mediates the transport of collagen to lysosomes for degradation. Its loss promotes accumulation of Collagen VI leading to nodule formation. Antrx2 -/- mice are shown to display massive accumulation of collagen VI in their uteri leading to fibrosis and sterility. It binds to collagen VI via its vWA domain (aa 44-213) in a MIDAS (metal ion-dependent binding domain) manner. (Ref.: Burgi, J., et al. (2017). Nat. Commun. 8; Article 15861; Chaudhary, A., et al. (2012). Cancer Cell. 21(2): 212-226).
Immunogen
A recombinant fragment of 178 amino acids from the N-terminal half of human ANTXR2 with an N-terminal extension of GSHMLEDPRG.
Application
Quality Control Testing
Evaluated by Western Blotting in Mouse lung tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ANTXR2/CMG-2 in Mouse lung tissue lysate.
Tested Applications
Western Blotting Analysis: A representative lot detected ANTXR2/CMG-2 in Western Blotting applications (Chaudhary, A., et al. (2012). Cancer Cell. 21(2):212-26; Szot, C., et al. (2018). J Clin Invest. 128(7):2927-2943).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1H8 is a mouse monoclonal antibody that detects Anthrax toxin receptor 2 (ANTXR2). It targets an epitope within the N-terminal half.
Physical form
Purified mouse monoclonal antibody IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
