General description
Envelope glycoprotein B (UniProt: P13201; also known as gB) is encoded by the gB (also known as UL55) gene (Gene ID: 3077424) in human cytomegalovirus (HHV-5). Human cytomegalovirus (HCMV), a member of the β-Herpesviridae family, is a major cause of morbidity and mortality in organ transplant recipients and patients with AIDS. Its envelope glycoprotein B is reported to be essential for the infectivity and is considered to be one of the most immunogenic virus-encoded proteins. It facilitates the entry of HCMV entry into cells by binding receptors and mediating membrane fusion. Glycoprotein B is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to produce the mature protein that contains a virion surface domain (aa 23-751), a transmembrane domain (aa 752-772), and an intra-virion domain (aa 773-907). The amino acid sequences 152-158 and 237-244 are critical for fusion and/or binding to host cell membrane. During virion morphogenesis, envelope glycoprotein B probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. It is then transported to the cell surface from where it is endocytosed and is directed to the trans-Golgi network. The ectodomain of HCMV glycoprotein B forms rosette-like aggregates that are similar to rosettes formed by the EBV glycoprotein B ectodomain. Four exposed hydrophobic residues in its fusion loop are shown to be essential for rosette formation. A linear domain of glycoprotein B, designated at AD-1, represents a dominant antibody binding site on this protein and is also required for oligomerization of glycoprotein B. (Ref.: Sharma, S., et al. (2013). Virology. 435(2); 239-249; Britt, WJ., et al. (2005). J. Virol. 79(7); 4066-4079; Jean, F., et al. (2000). Proc. Natl. Acad. Sci. USA. 97(6); 2864-2869; Speckner, ., et al. (1999). J. Gen. Virol. 80(8); 2183-2191; Schoppel, K., et al. (1996). Virology. 216(1); 133-145).
Immunogen
Whole Human cytomegalovirus (HCMV).
Application
Quality Control Applications
Evaluated by Western Blotting with recombinant HCMV glycoprotein B.
Western Blotting Analysis (WB): A 1:200,000 dilution of this antibody detected recombinant HCMV glycoprotein B.
Tested applications
Immunoprecipitation Analysis: A representative lot immunoprecipitated HCMV Envelope glycoprotein B in Immunoprecipitation applications (Reschke, M., et al. (1995). J Gen Virol. 76(Pt1); 113-22; Meyer, G.A., et al. (2000). J Biol Chem. 275(6); 3857-66; Vanarsdall, A.L., et al. (2008). J Virol. 82(23);11837-50).
Immunocytochemistry Analysis: A representative lot detected HCMV Envelope glycoprotein B in Immunocytochemistry applications (Reschke, M., et al. (1995). J Gen Virol. 76(Pt1); 113-22; Jean, F., et al. (2000). Proc Natl Acad Sci USA. 97(6); :2864-9; Meyer, G.A., et al. (2000). J Biol Chem. 275(6); 3857-66; Strive, T., et al. (2002). J Virol. 76(3); 1252-64).
Neutralizing: A representative lot neutralized Envelope glycoprotein B of HCMV. (Schoppel, K., et al. (1996). Virology. 216(1);133-45; Kropff, B., et al. (1997). J Gen Virol. 78(Pt8); 1999-2007; Vanarsdall, A.L., et al. (2008). J Virol. 82(23); 11837-50).
Immunofluorescence Analysis: A representative lot detected HCMV Envelope glycoprotein B in Immunofluorescence applications (Reschke, M., et al. (1995). J Gen Virol. 76(Pt1);113-22; Jean, F., et al. (2000). Proc Natl Acad Sci USA. 97(6); 2864-9; Meyer, G.A., et al. (2000). J Biol Chem. 275(6); 3857-66; Strive, T., et al. (2002). J Virol. 76(3); 1252-64; Vanarsdall, A.L., et al. (2008). J Virol. 82(23); 11837-50).
ELISA Analysis: A representative lot detected HCMV Envelope glycoprotein B in ELISA applications (Sharma, S., et al. (2013). Virology. 435(2); 239-49).
Western Blotting Analysis: A representative lot detected HCMV Envelope glycoprotein B in Western Blotting applications (Reschke, M., et al. (1995). J Gen Virol. 76(Pt1); 113-22; Schoppel, K., et al. (1996). Virology. 216(1); 133-45; Kropff, B., et al. (1997). J Gen Virol. 78(Pt8);1999-2007; Jean, F., et al. (2000). Proc Natl Acad Sci USA. 97(6); 2864-9; Sharma, S., et al. (2013). Virology. 435(2); 239-49; Khanh Le-Trilling, V. T., et al. (2020). Cell Rep. 30(7); 2248-2260).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 27-156 is a mouse monoclonal antibody that detects the envelope glycoprotein B in Human cytomegalovirus (HCMV). It targets an epitope within the antigenic domain 1 (AD1) within the C-terminal half.
Physical form
Purified mouse monoclonal antibody IgG2b in PBS without azide.
Preparation Note
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
