Description
General description
Dystroglycan (UniProt: Q62165; also known as Dystrophin-associated glycoprotein 1) is encoded by the Dag1 (also known as Dag-1) gene (Gene ID: 13138) in murine species. Dystroglycan is a component of the dystrophin glycoprotein complex that links the extracellular matrix to the intracellular actin cytoskeleton. It is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved off to generate the mature protein that is further autolytically cleaved (between 651-652) to produce alpha-dystroglycan (aa 28-651) and beta-dystroglycan (aa 652-893). Alpha-dystroglycan is an extracellular peripheral glycoprotein that binds to several extracellular matrix and synaptic proteins such as laminin, agrin, neurexin, and pikachurin. It is reported that its O-mannosyl glycosylation is required for its ligand-binding functions. Beta-dystroglycan is a single-pass type 1 transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton and serves as a cell adhesion receptor in both muscle and non-muscle tissues. In the cell, beta-dystroglycan binds to dystrophin that is linked to actin cytoskeleton. Hence, alpha/beta dystroglycans act as a molecular axis connecting extracellular matrix with the cytoskeleton across the plasma membrane. Defects in O-mannosyl glycan have been linked to various congenital muscular dystrophies caused by aberrant alpha-dystroglycan glycosylation. (Ref.: Sudo, A., et al. (2018). Human Mol. Genetics. 27(7); 7 1174-1185; Kanagawa, M., et al. (2010). J. Biol. Chem. 285(41); 31208-31216).
Immunogen
Murine alpha-dystroglycan-Fc-Fusion protein.
Application
Anti-Alpha Dystroglycan, clone 3D7, Cat. No. MABN2515, is a rat monoclonal antibody that detects Dystroglycan and has been tested for use in Western Blotting.
Western Blotting Analysis: A representative lot detected Alpha Dystroglycan in Western Blotting applications (Ohtsuka, Y., et. al. (2015). Sci Rep. 5:8316; Kanagawa, M., et. al. (2010). J Biol Chem. 285(41):31208-16; Kanagawa, M., et. al. (2016). Cell Rep
Analysis Note
Evaluated by Western Blotting in mouse brain tissue lysates.
Western Blotting Analysis: 1 ug/mL of this antibody detected Alpha Dystroglycan in mouse brain tissue lysates.
Other Notes
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
