Description
General description
Pancreas performs both the endocrine and the exocrine function. The bulk of pancreas is composed of exocrine cells that produce digestion enzymes which are released into ducts to be emptied into the duodenum. The endocrine pancreas is composed of islets of Langerhans that produce hormones. At least five distinct endocrine cell types have been identified in the endocrine pancreas, each producing a different hormone. Beta cells produce insulin, which is considered as an anabolic hormone and regulates blood glucose levels and is also involved in fat and protein synthesis. Alpha cells produce glucagon that acts when blood glucose levels are low. Ductal cells of pancreas form the epithelial lining of the branched tubes that deliver digestive enzymes into the duodenum. They also secrete bicarbonate to neutralize acidic contents coming in from stomach. Duct cells may contribute up to 10% of all pancreatic cells. Acinar cells function as a unit of the exocrine pancreas and they synthesize, store, and secrete digestive enzymes (lipase, alpha amylase, trypsin, and chymotrypsin), which under normal physiological conditions are activated only after reaching the duodenum. Premature activation of these enzymes within pancreatic acinar cells can lead to acute pancreatitis. Clone DHIC2-2B4 can be used to detect alpha cells in human pancreatic islets. (Ref.: Dorrell, C., et al. (2008). Stem Cell Research (2008). 1(3); 183-194; Grapin-Botton, A (2005). Int. J. Biochem. Cell Biol. 37(3); 504-510; Leung, PS (2006). Int. J. Biochem. Cell Biol. 38(7); 1024-1030).
Immunogen
Trypsin-dissociated human pancreatic islet cells containing low levels of exocrine and ductal cells.
Application
Detects Alpha cells of human pancreatic islets using this mouse monoclonal Anti-HPa2, clone DHIC2-2B4, Cat. No. MABS2045, tested for use in Immunohistochemistry (Paraffin) and Flow Cytometry.
Immunohistochemistry Analysis: A representative lot detected HPa2 in Immunohistochemistry applications (Dorrell, C., et. al. (2008). Stem Cell Res. 1(3):183-94).
Flow Cytometry Analysis: A representative lot detected HPa2 in Flow Cytometry applications (Dorrell, C., et. al. (2008). Stem Cell Res. 1(3):183-94; Bramswig, N.C., et. al. (2013). J Clin Invest. 123(3):1275-84; Dorrell, C., et. al. (2016). Nat Commun. 7:11756).
Biochem/physiol Actions
Clone DHIC2-2B4 specifically detects alpha cells in human pancreatic tissue sections.
Physical form
Format: Purified
Analysis Note
Evaluated by Immunohistochemistry (Paraffin) in alpha cells of human pancreatic tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected HPa2 in alpha cells of human pancreatic tissue sections.
Other Notes
Concentration: Please refer to lot specific datasheet.
