Description
General description
Acinar cells are the exocrine cells of the pancreas that synthesize and secrete digestive enzymes. Both synthesis and secretion of digestive enzymes are highly regulated over short term and long-time frames. To support their secretory function, acinar cells contain an abundant rough endoplasmic reticulum and Golgi apparatus. However, their size shrinks due to lack of luminal nutrients. Enzymes from acinar cells are released into a bicarbonate-rich solution secreted by the centroacinar and ductal cells and flows from the acini and acinar tubules to the intralobular ducts, then into the interlobular ducts and main duct, and finally into the duodenum at the major or minor papillae. Acinar cell growth is a major contributor to the postnatal pancreas growth min mice. Full development of acinar tissue extends into the postnatal period. In mice, pancreatic development that begins at E8.5 is largely completed by day W14.5. Acinar cells that are around islets are shown to be larger and more proliferative and it is suggested that islets communicate with adjacent acinar cells. When beta cell function is compromised in early stages of diabetes, acinar cell proliferation is reduced that lead to a gradual reduction in the size of the pancreas. Acinar cell volume is reported to vary about 9-fold among 24 mammalian species studied and exhibits an inverse correlation with the lifespan of the animal. Clone 2A6 is highly specific for acinar cells and can be used for isolation of these cells from pancreatic tissue. (Ref.: Anzi, S., et al. (2018). Developmental Cell 45(6); 726-737; Dorrell, C., et al. (2011). Mol. Cell. Endocrinol. 339(1-2); 144-150).
Immunogen
Purified murine pancreatic islets following preimmunization with partially dispersed whole pancreatic tissue, fetal bovine serum, and collagenase and cyclophosphamide treatment to eliminate B- lymphocytes reacting against these antigens.
Application
Quality Control TestingEvaluated by Immunohistochemistry in mouse pancreas tissue sections. Immunohistochemistry Applications: A 1:25 dilution of this antibody detected acinar cells in mouse pancreas tissue sections.Tested ApplicationsImmunofluorescence Analysis: A representative lot detected acinar cells in Immunofluorescence applications (Dorrell, C., et. al. (2011). Mol Cell Endocrinol. 339(1-2):144-50).Immunohistochemistry Applications: A representative lot detected acinar cells in Immunohistochemistry applications (Dorrell, C., et. al. (2011). Mol Cell Endocrinol. 339(1-2):144-50).Flow Cytometry Analysis: A representative lot detected acinar cells in Flow Cytometry applications (Dorrell, C., et. al. (2011). Mol Cell Endocrinol. 339(1-2):144-50; Anzi, S., et. al. (2018). Dev Cell. 45(6):726-737).Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Acinar cells, clone MIC0-2A6, Cat. No. MABS2144, is a rat monoclonal antibody that detects acinar cells and is tested for use in Flow Cytometry, Immunofluorescence, and Immunohistochemistry.
Biochem/physiol Actions
Clone MIC0-2A6 is a rat monoclonal antibody that detects murine acinar cells.
Physical form
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at +2°C to +8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
