Description
General description
Cytochrome b-245 heavy chain (UniProt: P04839; also known as CGD91-phox, Cytochrome b(558) subunit beta, Cytochrome b558 subunit beta, Heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, Neutrophil cytochrome b 91 kDa polypeptide, Superoxide-generating NADPH oxidase heavy chain subunit, gp91-1, gp91-phox, p22 phagocyte B-cytochrome) is encoded by the CYBB (also known as NOX2) gene (Gene ID: 1536) in Human. Gp91phox is a multi-pass membrane glycoprotein of the NADPH oxidase family that contains three extracellular domains, six transmembrane domains, and four cytoplasmic domains. It contains four iron binding sites and has a ferric oxidoreductase domain that is localized in the amino acids 54-286. It is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide and plays an important role in the host defense function of phagocytic cells. It also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes. It can undergo polyubiquitination at lysine 48 by RNF145 that triggers its endoplasmic reticulum-associated degradation. Clone NL7 binds to an epitope in the C-terminal region, but it does not inhibit the generation of superoxide. Mutations in CYBB gene are known to cause granulomatous disease that is characterized by the inability of neutrophils and phagocytes to kill ingested leading to life-threatening bacterial and fungal infections. (Ref.: (Ref.: Burritt, JB., et al. (2003). J. Immunol. 170 (12); 6082-6089).
Immunogen
Purified human neutrophil flavocytochrome b588 (gp91phox).
Application
Quality Control Testing
Evaluated by Western Blotting in Human PMN cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected gp91phox (NOX2) in Human PMN cell lysate.
Tested Applications
Inhibition Analysis: A representative lot detected gp91phox (NOX2) in Inhibition applications (Burritt, J.B., et. al. (2003). J Immunol. 170(12):6082-9).
Western Blotting Analysis: A representative lot detected gp91phox (NOX2) in Western Blotting applications (Burritt, J.B., et. al. (2003). J Immunol. 170(12):6082-9).
Flow Cytometry Analysis: A representative lot detected gp91phox (NOX2) in Flow Cytometry applications (Burritt, J.B., et. al. (2003). J Immunol. 170(12):6082-9).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-gp91phox (NOX2), clone NL7, Cat. No. MABS2193, is a mouse monoclonal antibody that detects Cytochrome b-245 heavy chain (gp91phox) and is tested for use in Flow Cytometry, Inhibition Analysis, and Western Blotting.
Biochem/physiol Actions
Clone NL7 is a mouse monoclonal antibody that detects gp91-phox/NOX2. It targets an epitope within 10 amino acids from the C-terminal, fourth cytoplasmic domain.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing PBS without azide
Preparation Note
Storage and Handling Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/ thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
