Merck, MABT1565-25UG, Anti-Desmin Antibody, clone DE-U-10

General description
Desmin (UniProt: P02540) is encoded by the DES gene (Gene ID: 396725) in porcine. Desmin is a type III intermediate filament protein that is essential for maintaining the structural and functional integrity of muscle cells as part of the cytoskeletal network. It provides mechanical support to the muscle cells and facilitates the organization of contractile proteins within the cell. Additionally, it is involved in various cellular processes, including cell signaling, maintenance of cell shape, and intracellular transport. It is particularly abundant in cardiac, skeletal, and smooth muscle tissues. It is also enriched at postsynaptic membranes of neuromuscular junctions (NMJs). Mutations in the gene encoding Desmin have been associated with hereditary and sporadic myopathies, cardiomyopathies and myasthenic syndrome. (Ref.: Eiber, N., et al., (2020). Front. Mol. Neurosci. 13; Article 567084).
Immunogen
Purified full length porcine Desmin.
Application
Quality Control Testing

Evaluated by Western Blotting in mouse skeletal muscle tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Desmin in mouse skeletal muscle tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Desmin in human skeletal muscle tissue extracts.

ELISA Analysis: A representative lot detected Desmin in ELISA applications (Debus, E., et al. (1983). EMBO J. 2(12):2305-12).

Western Blotting Analysis: A representative lot detected Desmin in Western Blotting applications (Debus, E., et al. (1983). EMBO J. 2(12):2305-12).

Immunofluorescence Analysis: A representative lot detected Desmin in Immunofluorescence applications (Debus, E., et al. (1983). EMBO J. 2(12):2305-12).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone DE-U-10 is a mouse monoclonal antibody that detects Desmin. It targets an epitope within 48 amino acids from the C-terminal half of the protein.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.