Merck, MAK610-1KT, Chymotrypsin Inhibitor Assay Kit

General description
Chymotrypsin(EC 3.4.21.1) is a digestive, serine protease that hydrolyzes dietary proteinsin many eukaryotic and prokaryotic organisms. Chymotrypsin predominantlycleaves peptide chains at the carboxyl side of the aromatic phenylalanine,tryptophan, and tyrosine amino acids.
Application
For the evaluation of drugs and screening of potential inhibitors to chymotrypsin proteases.
Biochem/physiol Actions
TheChymotrypsin Inhibitor Assay Kit uses a fluorescein isothiocyanate(FITC)-labeled synthetic substrate. The fluorescein label is highly quenched.Upon digestion by chymotrypsin present in the sample, the substrate is cleavedinto smaller peptides, which abolishes the quenching of the fluorescence label.The fluorescence or fluorescence polarization (FP) of the FITC-labeledfragments is measured at λex/em = 485/530 nm. Inhibition is determined by thedecrease in fluorescence.