Merck, O4000-1G, Oxythiamine chloride hydrochloride

General description
Oxythiamine is a thiamine antimetabolite and the source through diet is by consuming acidic thiamine rich foods.
Application
Oxythiamine chloride hydrochloride is suitable for use:
as a reference standard for calibration curve generation in liquid chromatography-tandem mass spectrometry (LC-MS/MS) for oxythiamine pyrophosphate (OTPP) quantification in red blood cells
as a thiamine transport inhibitor in human mammary epithelial cells (HMEC) and breast cancer cell lines
in the synthesis of oxythiamineH picrolonate salt

Biochem/physiol Actions
Oxythiamine (OT) is a thiamine antagonist. It is a transketolase inhibitor and is employed to study anti-metastatic mechanisms, especially those involving metalloproteinases (MMP). It significantly sensitizes human hepatocellular carcinoma cells (HCC) to sorafenib, favoring tumor suppression.
Oxythiamine inhibits Transketolase, the enzyme that controls the nonoxidative branch of the pentose phosphate pathway.