Merck, O4387-.1ML, Oligo(dT)23, Anchored

General description
Oligo(dT)23, Anchored primers are used to prime mRNA with a poly(A) tail for cDNA synthesis. The primers have 23 thymidine residues and one G, C, or A residue (the anchor) at the 3′ end. This anchor ensures that the oligo(dT) primer binds at the very beginning of the message and there is not a long region of the unusable sequence. The optimal conditions for the concentration of enhanced AMV reverse transcriptase, template RNA, primers, and amplification parameters will depend on the system being utilized and should be determined empirically.
Application
Oligo(dT)23, Anchored has been used in the synthesis of cDNA for real-time quantitative polymerase chain reaction (PCR).
The Anchored Oligo(dT)₂₃Primers have 23 thymidine residues and one G, C, or A residue (the anchor) at the 3′ end. This anchor ensures the oligo(dT)₂₃ primer binds at the beginning of the message such that there are no long regions of unusable sequence. Anchored oligo(dT)₂₃ primers may provide an advantage over standard oligo(dT) primers when generating cDNA from poly(A)+ RNA.
Features and Benefits
In the process of preparing cDNA libraries, when there is incomplete or missing sequence information or when specific primers are not suitable, Oligo(dT)23, Anchored primers can be employed together with random nonamers (Product No. R 7647).
These primers can serve as a substitute for reverse transcription primers in tasks like first-strand cDNA synthesis, cDNA library construction, and various other applications.
Following transcription, the anchored oligo(dT)23 primers exhibit a reduced ability to initiate priming at higher temperatures (up to 65 °C), ensuring that they do not interfere with PCR.
Anchored oligo(dT)23 primers may provide an advantage over standard oligo(dT) primers when generating cDNA from poly(A)+ RNA.

Other Notes
For laboratory use only. Not for drug, household or other uses.