Description
General description
JumpStart REDTaq ReadyMix PCR Reaction Mix for High Throughput PCR is formulated with REDTaq Genomic DNA polymerase for amplification of more complex templates and genomic templates. This mix contains optimized enzyme and dye concentrations to provide increased length and yield on the more difficult templates.
JumpStart™ REDTaq® ReadyMix™PCR Reaction Mix is a prepared solution combining the performance benefits of hot start PCR with the dual convenience of Sigma′s ReadyMix and REDTaq. The Master mix includes Sigma′s JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer, and an inert red dye in a 2× optimized reaction concentrate. Add 25 μL of the ReadyMix to primers, template, and water to a final reaction volume of 50 μL. JumpStart Taq antibody in the reaction mix inactivates the Taq DNA polymerase at room temperature. During the first denaturation step of PCR the complex dissociates and polymerase activity is fully restored. The inert red dye does not effect automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. The PCR product is easily separated from the dye by standard purification methods if desired. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications.
Application
JumpStart™REDTaq®ReadyMix™ Reaction Mix has been used:
For multiplex PCR
For amplification with reduced non-specific amplification
For high throughput PCR amplification of complex templates
In the amplification of primers developed for the pathogenicity-associated genes of Clavibacter michiganensis
For PCR amplification of the human TMPRSS6 gene
Features and Benefits
JumpStart™ REDTaq® DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
JumpStart Taq DNA polymerase provides superior amplification regardless of template concentration
REDTaq JumpStart ReadyMix reduces pipetting steps and risk of contamination. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications
Inert red dye allows for easy verification that reagent has been mixed properly
Other loading dyes are not required. Aliquots may be directly loaded onto an agarose gel after the reaction
Packaging
Default reaction volume is 50 μL
20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
800RXN is packaged as 1 X 20 mL
Other Notes
One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74 °C.
View more detailed information on JumpStart REDTaq and ReadyMix products at www.sigma-aldrich.com/hotstart.
Legal Information
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
