Merck, P4404-5MG, Polyguanylic acid potassium salt

General description
Polyguanylic acid takes up a four-stranded helical structure. It is synthetically produced in Thermus thermophilus from guanosine diphosphate (GDP) and its synthesis is catalyzed by the enzyme polynucleotide phosphorylase.
Application
Polyguanylic acid (PolyG) is used to study the effects of ionizing radiation on formation and stability of G-quadruplex structures and as a target molecule for physicochemical studies of electronic excitation (vibrational spectra) of guanosine.
Polyguanylic acid potassium salt has been used:
as a ligand for surface neuropilin-1 (NRP1) for internalization studies
for intercalation studies with trisubstituted and disubstituted triazole-linked phenyl derivatives CL41, CL42 and CL2r50 using voltammetry method
as a synthetic polynucleotide for agarose gel electrophoresis analysis

Biochem/physiol Actions
Polyguanylic acid acts as a ligand for scavenger receptor and reduces the surface expression neuropilin-1 in endothelial cells.
Preparation Note
Prepared from GDP using polynucleotide phosphorylase