Description
General description
Phosphate-citrate buffer is a common buffer used along with soluble horseradish peroxidase substrates such as o-phenylenediaminedihydrochloride (OPD) or tetramethylbenzidine (TMB). It gives a pH of 5.0 with an excellent buffering capacity. The phosphate-citrate buffer tablets can be used to prepare a 0.05 M phosphate-citrate, pH 5.0, buffer solution. These tablets elicit a fast, convenient, and accurate method for the synthesis of phosphate citrate buffer solutions. They remove the time-consuming and tedious process of weighing individual components in buffer preparation. Phosphate-citrate buffer tablets have been manufactured from the highest quality components to exacting physical and chemical specifications to ensure performance and lot-to-lot consistency. Phosphate-Citrate Buffer tablets, pH 5.0, have been formulated as a substrate buffer in immunoassay procedures.
Application
Characterization of nettle leaves (Urtica dioica) as a novel source of protease for clotting dromedary milk by non-destructive methods.: This study uses phosphate-citrate buffer in the enzymatic extraction processes to maintain stability and activity of plant-derived enzymes, demonstrating its crucial role in the development of natural coagulants for dairy products (Bouazizi et al., 2022).
A rapid, low pH, nutrient stress, assay to determine the bactericidal activity of compounds against non-replicating Mycobacterium tuberculosis.: Utilizing phosphate-citrate buffer, this study develops a low pH nutrient stress assay for evaluating the efficacy of antibacterial agents against dormant tuberculosis bacteria, providing a critical tool for drug development (Early et al., 2019).
Measurement of Free Iodine in Different Formulations of Povidone-Iodine Eye Drops 5.: This investigation uses phosphate-citrate buffer to measure the stability and release of iodine in ophthalmic solutions, highlighting its importance in ensuring the efficacy and safety of eye care products (Prado et al., 2019).
Preparation Note
One tablet dissolved in 100 mL deionized water yields a 0.05 M phosphate-citrate buffer, pH 5.0, at 25 °C. Store at room temperature.
