Description
General description
The Protease Inhibitor Cocktail for fungal and yeast extracts is a mixture of protease inhibitors in DMSO solution. The cocktail has been optimized and tested for use on fungal and yeast samples, specifically on Saccharomyces cerevisiae cells. One mL of the cocktail is recommended for the inhibition of proteases extracted from 20 g of yeast.
Application
Protease Inhibitor Cocktail has been used as a component in:
FA buffer to wash spheroplasts to obtain an average DNA fragment size for chromatin immunoprecipitation (chip)
potassium phosphate buffer (pbs) to digest chitin
extraction buffer for grinding powdered mycelia for co-immunoprecipitation analysis
Biochem/physiol Actions
Inhibits serine, cysteine, aspartic, and metalloproteases.
Features and Benefits
Broad specificity: inhibits a wide range of proteases, providing comprehensive protection to fungal and yeast extracts.
Tested on Saccharomyces cerevisiae cells: the cocktail has been optimized for use on this commonly studied yeast strain.
Convenient packaging: available in a 1 or 5 mL glass bottle for easy handling and storage.
Ready-to-use solution: the cocktail is supplied in DMSO solution for immediate use in protease inhibition assays.
Effective inhibition: each component in the cocktail has been carefully selected for its specific inhibitory properties, ensuring reliable and consistent results.
Physical form
Solution in DMSO
Preparation Note
One mL is recommended for the inhibition of proteases extracted from 20 g of yeast.
Other Notes
AEBSF, 100 mM
E-64, 1.4 mM
Pepstatin A, 2.2 mM
1,10-Phenanthroline, 500 mM
Mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic and metallo-proteases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatin A, E-64, and 1,10-phenanthroline.
This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
