Description
General description
RNAlater is compatible with most RNA isolation methods, including TRI Reagent and GenElute Total RNA isolation and mammalian mRNA isolation kits.
RNAlater is an aqueous, non-toxic tissue storage reagent that rapidly permeates tissue to stabilize and protect cellular RNA in situ in unfrozen specimens. Tissue pieces are harvested and immediately submerged in RNAlater for storage without jeopardizing the quality or quantity of RNA. RNAlater eliminates the need to immediately process tissue specimens or to freeze samples in liquid nitrogen for later processing. RNAlater preserves RNA in tissues for up to 1 day at 37 °C, 1 week at 25 °C, and 1 month at 4 °C. Tissues can also be stored at -20 °C long-term.
RNAlater has been extensively tested on several tissues from vertebrate species, including brain, heart, kidney, spleen, liver, testis, skeletal muscle, fat, lung, and thymus. RNAlater is also effective for E. coli, Drosophila, tissue culture cells, white blood cells, and some plants.
RNAlater can be used with various downstream applications including mRNA and total RNA isolation, histology and immunocytochemistry and is compatible with Sigma′s isolation kits.
Application
RNAlater® has been used to synthesize antisense RNA probe. It can be used with various downstream applications including mRNA and total RNA isolation, histology and immunocytochemistry and is compatible with Sigma′s GenElute™ isolation kits.
Biochem/physiol Actions
RNAlater is easy to use. Simply cut tissue samples to be stored so they are less than 0.5 cm in at least one dimension and submerge in 5 volumes of RNAlater. Small organs, such as rat kidney, liver or spleen can be stored in whole in RNAlater. When ready to isolate the RNA, remove the tissue from RNAlater and process as though just harvested. For cell storage, resuspend pelleted cells in a small amount of PBS before adding 5-10 volumes of RNAlater. Before preparing RNA, pellet cells and discard supernatant.
Features and Benefits
No compromise in RNA quality following mRNA or total RNA isolation
Rapidly permeates tissues to stabilize and protect cellular RNA
Aqueous non-toxic solution allows downstream tissue processing
Stabilizes samples at room temperature
Other Notes
RNAlater® is an aqueous, non-toxic tissue and cell storage reagent that stabilizes and protects cellular RNA in intact, unfrozen tissue and cell samples. RNAlater eliminates the need to immediately process samples or to freeze samples in liquid nitrogen for later processing.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
RNAlater is a registered trademark of Thermo Fisher Scientific, Inc.
