Merck, R106-05N, Rat Dermal Fibroblasts: RDF, neonatal

General description
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RDF from Cell Applications, Inc. have been used in allogenic prosthetic grafts, demonstrating improved epithelization, collagen organization and faster regeneration (Nomoto, 2012), in a cell sheet engineering study, where a sheet of dermal fibroblasts was transplanted into the damaged liver in allogeneic rat model to promote migration of hepatic progenitor cells and faster healing (Muraoka, 2013), and as a control in engineered cardiac tissues (Serrao, 2012).
Application
Injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response.
Biochem/physiol Actions
Skin
Preparation Note
1st passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO
Can be cultured at least 8 population doublings

Please refer to the RDF Culture Protocol.
Analysis Note
Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.
Other Notes
Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO