Merck, R2283-15ML, Pepsin Reagent, Ready to Use, Antigen Retriever

General description
Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to the application of antibodies. In immunohistochemistry (IHC), the most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. Pepsin digestion of FFPE tissue sections improves the accessibility of antibodies to tissue antigens. Ready to use pepsin reagent, antigen retriever has been used for antigen unmasking in immunohistochemistry.
Application
Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to the application of antibodies in immunohistochemistry (IHC).It has been used to enhance the permeabilization of fungal cell walls during the fluorescence in situ hybridization (FISH) process and utilized during the RNA scope in situ hybridization process to perform antigen retrieval.