Merck, R4752-100UN, Ribonucleic acid, transfer from bovine liver

General description
Transfer ribonucleic acid (tRNA) is a non-coding RNA. It is approximately 70-100 base pair long. It has a cloverleaf shaped secondary structure. It has three or four arms and a stem structure. It has minor bases like pseudouridine, dihydrouridine and methylated bases in its structure. It has a binding site for triplet nucleotide sequence and for amino acids.
Application
Ribonucleic acid, transfer from bovine liver may be used as a starting material for the purification of specific amino acyl-tRNA species by methods such as liquid column chromatography or high-performance liquid chromatography (HPLC).
Ribonucleic acid, transfer from bovine liver has been used:
to analyze the efficiency of thioflavin T (ThT) as a fluorophore
to test its ability to inhibit the growth of endothelial cells
as a component in translation mixture
as a non-specific competitor RNA for protein purification studies using poly(A) tailed RNA affinity column

Biochem/physiol Actions
Transfer ribonucleic acid (tRNA) plays a major role in translation, a key step in protein synthesis. It decodes mRNA into protein. There are about 40-60 different tRNAs in a eukaryotic cell and fewer in bacteria. This depends on the degeneracy of the amino acid code. Each amino acid has a specific tRNA. tRNAs are charged with specific amino acid and carries them to the site of protein synthesis. It binds to the ribosome and directs the amino acid to the growing polypeptide chain. It also functions as a regulator of various biological processes. Mutations associated with tRNA is implicated in human diseases.
Analysis Note
This product is evaluated for amino acceptor activity using aminoacyl-tRNA synthetase and L-arginine
Other Notes
One unit will yield an A260 of 1.0 in 1.0 mL of water (1 cm light path).